Mycoparasitic fungi reducing the incidence and virulence of Bipolaris sorokiniana (2013)

The mycoparasitic efficiency of 28 strains/isolates of Clonostachys rosea f. rosea and Clonostachys rosea f. catenulata against the pathogenic fungus Bipolaris sorokiniana was determined in dual cultures on Czapek-Dox nutrient agar. Strains with low and medium efficiency were antagonistically inhibited by B. sorokiniana, and inhibitory zones were formed between the colonies of both fungi. The mycelium of Clonostachys strains with high efficiency overgrew and degraded B. sorokiniana colonies without formation of an inhibitory zone. In dual cultures, Trichoderma sp. and the most effective Clonostachys strains degraded B. sorokiniana colonies within 3 to 5 and 12 to 15 days, respectively. When rye seeds were treated with a mixture of C. rosea f. rosea, Trichoderma sp. and B. sorokiniana, development of B. sorokiniana on both seeds and seedlings was reduced when compared with a B. sorokiniana treatment. The treatment of rye seeds with Clonostachys and Trichoderma had a positive effect on seed germination and seedling length.

CZECH MYCOLOGY. 2013,  65(1): 103–112, JUNE 10. (ONLINE VERSION, ISSN 1805-1421)